{"id":515,"date":"2024-11-29T15:39:23","date_gmt":"2024-11-29T15:39:23","guid":{"rendered":"https:\/\/www.asteasier.com\/old\/?page_id=515"},"modified":"2024-11-29T15:41:12","modified_gmt":"2024-11-29T15:41:12","slug":"scientific-publications","status":"publish","type":"page","link":"https:\/\/www.asteasier.com\/old\/scientific-publications\/","title":{"rendered":"Publications"},"content":{"rendered":"<section class=\"wpb-content-wrapper\"><p>[vc_row css_animation=&#8221;&#8221; row_type=&#8221;row&#8221; use_row_as_full_screen_section=&#8221;no&#8221; type=&#8221;full_width&#8221; angled_section=&#8221;no&#8221; text_align=&#8221;left&#8221; background_image_as_pattern=&#8221;without_pattern&#8221;][vc_column]\n\t\t\t<!-- START scientific publications REVOLUTION SLIDER 6.6.8 --><p class=\"rs-p-wp-fix\"><\/p>\n\t\t\t<rs-module-wrap id=\"rev_slider_7_1_wrapper\" data-source=\"gallery\" style=\"visibility:hidden;background:transparent;padding:0;margin:0px auto;margin-top:0;margin-bottom:0;\">\n\t\t\t\t<rs-module id=\"rev_slider_7_1\" style=\"\" data-version=\"6.6.8\">\n\t\t\t\t\t<rs-slides style=\"overflow: hidden; position: absolute;\">\n\t\t\t\t\t\t<rs-slide style=\"position: absolute;\" data-key=\"rs-7\" data-title=\"Slide\" data-in=\"o:0;\" data-out=\"a:false;\">\n\t\t\t\t\t\t\t<img decoding=\"async\" src=\"\/\/www.asteasier.com\/old\/wp-content\/plugins\/revslider\/public\/assets\/assets\/dummy.png\" alt=\"\" title=\"header-generic-bg\" width=\"1920\" height=\"500\" class=\"rev-slidebg tp-rs-img rs-lazyload\" data-lazyload=\"\/\/www.asteasier.com\/old\/wp-content\/uploads\/2023\/02\/header-generic-bg.jpg\" data-no-retina>\n<!--\n\t\t\t\t\t\t\t--><rs-layer\n\t\t\t\t\t\t\t\tid=\"slider-7-slide-7-layer-0\" \n\t\t\t\t\t\t\t\tdata-type=\"shape\"\n\t\t\t\t\t\t\t\tdata-rsp_ch=\"on\"\n\t\t\t\t\t\t\t\tdata-xy=\"xo:50px,37px,31px,0;yo:50px,37px,31px,0;\"\n\t\t\t\t\t\t\t\tdata-text=\"w:normal;s:20,15,12,7;l:0,18,15,9;\"\n\t\t\t\t\t\t\t\tdata-dim=\"w:300px,225px,194px,100%;h:180px,135px,116px,100%;\"\n\t\t\t\t\t\t\t\tdata-vbility=\"f,f,f,t\"\n\t\t\t\t\t\t\t\tdata-basealign=\"slide\"\n\t\t\t\t\t\t\t\tdata-frame_999=\"o:0;st:w;\"\n\t\t\t\t\t\t\t\tstyle=\"z-index:6;background-color:rgba(0,0,0,0.5);\"\n\t\t\t\t\t\t\t><rs-bg-elem style=\"background: url('\/\/www.asteasier.com\/old\/wp-content\/uploads\/2023\/02\/Raggruppa-35.jpg') no-repeat center center; background-size: cover;\"><\/rs-bg-elem>\n \n\t\t\t\t\t\t\t<\/rs-layer><!--\n\n\t\t\t\t\t\t\t--><h1\n\t\t\t\t\t\t\t\tid=\"slider-7-slide-7-layer-1\" \n\t\t\t\t\t\t\t\tclass=\"rs-layer\"\n\t\t\t\t\t\t\t\tdata-type=\"text\"\n\t\t\t\t\t\t\t\tdata-color=\"#dd1a1e\"\n\t\t\t\t\t\t\t\tdata-rsp_ch=\"on\"\n\t\t\t\t\t\t\t\tdata-xy=\"xo:0,19px,19px,19px;y:m;\"\n\t\t\t\t\t\t\t\tdata-text=\"w:normal;s:72,54,46,42;l:80,60,51,44;fw:500;a:left,left,left,center;\"\n\t\t\t\t\t\t\t\tdata-dim=\"w:568px,426px,368px,446px;\"\n\t\t\t\t\t\t\t\tdata-frame_1=\"sR:690;\"\n\t\t\t\t\t\t\t\tdata-frame_999=\"o:0;st:w;\"\n\t\t\t\t\t\t\t\tstyle=\"z-index:7;font-family:'Montserrat';\"\n\t\t\t\t\t\t\t>Scientific publications \n\t\t\t\t\t\t\t<\/h1><!--\n-->\t\t\t\t\t\t<\/rs-slide>\n\t\t\t\t\t<\/rs-slides>\n\t\t\t\t<\/rs-module>\n\t\t\t\t<script>\n\t\t\t\t\tsetREVStartSize({c: 'rev_slider_7_1',rl:[1240,1024,778,480],el:[400,400,400,350],gw:[1200,900,778,480],gh:[400,400,400,350],type:'hero',justify:'',layout:'fullwidth',mh:\"0\"});if (window.RS_MODULES!==undefined && window.RS_MODULES.modules!==undefined && window.RS_MODULES.modules[\"revslider71\"]!==undefined) {window.RS_MODULES.modules[\"revslider71\"].once = false;window.revapi7 = undefined;if (window.RS_MODULES.checkMinimal!==undefined) window.RS_MODULES.checkMinimal()}\n\t\t\t\t<\/script>\n\t\t\t<\/rs-module-wrap>\n\t\t\t<!-- END REVOLUTION SLIDER -->\n[\/vc_column][\/vc_row][vc_row css_animation=&#8221;&#8221; row_type=&#8221;row&#8221; use_row_as_full_screen_section=&#8221;no&#8221; type=&#8221;grid&#8221; angled_section=&#8221;no&#8221; text_align=&#8221;left&#8221; background_image_as_pattern=&#8221;without_pattern&#8221; z_index=&#8221;&#8221; css=&#8221;.vc_custom_1732893113592{padding-top: 100px !important;padding-bottom: 100px !important;}&#8221;][vc_column]<div class=\"qode-accordion-holder clearfix qode-accordion qode-initial \">\n\t<h2 class=\"clearfix qode-title-holder\">\n<span class=\"qode-tab-title\">\n\t\t\t<span class=\"qode-tab-title-icon\">\n\t\t\t<i class=\"qode_icon_font_awesome fa fa-external-link-square \" ><\/i>\t\t<\/span>\n\t    <span class=\"qode-tab-title-inner\">\n        Genetic engineering of Nannochloropsisoceanica to produce canthaxanthinand ketocarotenoids    <\/span>\n<\/span>\n<span class=\"qode-accordion-mark\">\n    <span class=\"qode-accordion-mark-icon\">\n        <span class=\"icon_plus\"><\/span>\n        <span class=\"icon_minus-06\"><\/span>\n    <\/span>\n<\/span>\n<\/h2>\n<div id = toggle-bar class=\"qode-accordion-content qode-acc-title-with-icon\" >\n    <div class=\"qode-accordion-content-inner\">\n        [vc_column_text]<strong>Authors<\/strong><br \/>\nDavide Canini, Flavio Martini, Stefano Cazzaniga, Tea Miotti, Beatrice Pacenza, Sarah D\u2019Adamo &amp; Matteo Ballottari<\/p>\n<p><strong>Published<\/strong><br \/>\n29 November 2024<\/p>\n<p><strong>Journal<\/strong><br \/>\nMicrobial Cell Factories volume 23, Article number: 322 (2024)<\/p>\n<p><strong>DOI<\/strong><br \/>\n<a href=\"https:\/\/doi.org\/10.1186\/s12934-024-02599-4\">https:\/\/doi.org\/10.1186\/s12934-024-02599-4<\/a><\/p>\n<p><strong>Abstract<br \/>\n<\/strong>Background Canthaxanthin is a ketocarotenoid with high antioxidant activity, and it is primarily produced bymicroalgae, among which Nannochloropsis oceanica, a marine alga widely used for aquaculture. In the last decade,N. oceanica has become a model organism for oleaginous microalgae to develop sustainable processes to producebiomolecules of interest by exploiting its photosynthetic activity and carbon assimilation properties. N. oceanicacan accumulate lipids up to 70% of total dry weight and contains the omega-3 fatty acid eicosapentaenoic acid(EPA) required for both food and feed applications. The genome sequence, other omics data, and synthetic biologytools are available for this species, including an engineered strain called LP-tdTomato, which allows homologousrecombination to insert the heterologous genes in a highly transcribed locus in the nucleolus region. Here, N.oceanica was engineered to induce high ketocarotenoid and canthaxanthin production.<\/p>\n<p><strong>Results<br \/>\n<\/strong>We used N. oceanica LP-tdTomato strain as a background to express the key enzyme for ketocarotenoidproduction, a \u03b2-carotene ketolase (CrBKT) from Chlamydomonas reinhardtii. Through the LP-tdTomato strain, thetransgene insertion by homologous recombination in a highly transcribed genomic locus can be screened bynegative fluorescence. The overexpression of CrBKT in bkt transformants increased the content of carotenoidsand ketocarotenoids per cell, respectively, 1.5 and 10-fold, inducing an orange\/red color in the bkt cell cultures.Background (LP) and bkt lines productivity were compared at different light intensities from 150 to 1200 \u03bcmol m-2s-1: at lower irradiances, the growth kinetics of bkt lines were slower compared to LP, while higher productivity wasmeasured for bkt lines at 1200 \u03bcmol m-2 s-1. Despite these results, the highest canthaxanthin and ketocarotenoidsproductivity were obtained upon cultivation at 150 \u03bcmol m-2 s-1.<\/p>\n<p><strong>Conclusions<br \/>\n<\/strong>Through targeted gene redesign and heterologous transformation, ketocarotenoids and canthaxanthincontent were significantly increased, achieving 0.3% and 0.2% dry weight. Canthaxanthin could be produced usingCO2 as the only carbon source at 1.5 mg\/L titer. These bkt-engineered lines hold potential for industrial applications infish or poultry feed sectors, where canthaxanthin and ketocarotenoids are required as pigmentation agents.[\/vc_column_text]    <\/div>\n<\/div><\/div>[vc_empty_space height=&#8221;30px&#8221;][\/vc_column][\/vc_row]<\/p>\n<\/section>","protected":false},"excerpt":{"rendered":"<p>[vc_row css_animation=&#8221;&#8221; row_type=&#8221;row&#8221; use_row_as_full_screen_section=&#8221;no&#8221; type=&#8221;full_width&#8221; angled_section=&#8221;no&#8221; text_align=&#8221;left&#8221; background_image_as_pattern=&#8221;without_pattern&#8221;][vc_column][\/vc_column][\/vc_row][vc_row css_animation=&#8221;&#8221; row_type=&#8221;row&#8221; use_row_as_full_screen_section=&#8221;no&#8221; type=&#8221;grid&#8221; angled_section=&#8221;no&#8221; text_align=&#8221;left&#8221; background_image_as_pattern=&#8221;without_pattern&#8221; z_index=&#8221;&#8221; css=&#8221;.vc_custom_1732893113592{padding-top: 100px 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